open it,there are lots of programs that enable you to see whats inside
tbh,it must have vobs in it,cause i never seen a "film" iso that hasnt,but someone may say otherwise
i use isobuster or ultraiso
anyways,have you tried any other disks?,maybe a DVD+R type disk that has been booktyped to dvd-rom
also if you can,try it on another dvd player,maybe a game console if you have one
What type of files are in the ISO file?
Almost any ZIP program can open it.
If you created it, you should know what you put into it.
What program did you use to burn the image file?
What type of media?
What was the burning speed?